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验证数据展示

  • WB analysis using 67566-1-Ig

    WB analysis using 67566-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human urothelial carcinoma using 67566-1-Ig

    IHC staining of human urothelial carcinoma using 67566-1-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPS14 antibody included
  • WB analysis using 67566-1-Ig

    WB analysis using 67566-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67566-1-Ig

    WB analysis of HeLa using 67566-1-Ig

    WB result of RPS14 antibody (67566-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS14 transfected HeLa cells.

  • IF Staining of HepG2 using 67566-1-Ig

    IF Staining of HepG2 using 67566-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS14 antibody (67566-1-Ig, Clone: 3D5H5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

RPS14 Monoclonal antibody

RPS14 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67566-1-Ig

产品说明书

CloneNo. 3D5H5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

RPS 14, ribosomal protein S14, PRO2640, EMTB, 40S ribosomal protein S14

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, HuH-7 cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPS14 Ready-To-Use IHC Kit. RPS14 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67566-1-Ig targets RPS14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPS14 fusion protein Ag30032 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein S14
别名 RPS 14, ribosomal protein S14, PRO2640, EMTB, 40S ribosomal protein S14
计算分子量 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC003401
基因名称 RPS14
Gene ID (NCBI) 6208
RRIDAB_2882780
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62263
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].

实验方案

Product Specific Protocols
WB protocol for RPS14 antibody 67566-1-IgDownload protocol
IHC protocol for RPS14 antibody 67566-1-IgDownload protocol
IF protocol for RPS14 antibody 67566-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols