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验证数据展示

  • WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

    IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS14 antibody included
  • WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67566-1-Ig (same clone as 67566-1-PBS)

    WB analysis of HeLa using 67566-1-Ig (same clone as 67566-1-PBS)

    WB result of RPS14 antibody (67566-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS14 transfected HeLa cells. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67566-1-Ig (same clone as 67566-1-PBS)

    IF Staining of HepG2 using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS14 antibody (67566-1-Ig, Clone: 3D5H5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RPS14 Monoclonal antibody, PBS Only

RPS14 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67566-1-PBS

产品说明书

CloneNo. 3D5H5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RPS 14, ribosomal protein S14, PRO2640, EMTB, 40S ribosomal protein S14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RPS14 Ready-To-Use IHC Kit. RPS14 primary antibody included.

产品信息

67566-1-PBS targets RPS14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPS14 fusion protein Ag30032 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein S14
别名 RPS 14, ribosomal protein S14, PRO2640, EMTB, 40S ribosomal protein S14
计算分子量 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC003401
基因名称 RPS14
Gene ID (NCBI) 6208
RRIDAB_2882780
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62263
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].