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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

    IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS14 antibody included
  • WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67566-1-Ig (same clone as 67566-1-PBS)

    WB analysis of HeLa using 67566-1-Ig (same clone as 67566-1-PBS)

    WB result of RPS14 antibody (67566-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS14 transfected HeLa cells. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67566-1-Ig (same clone as 67566-1-PBS)

    IF Staining of HepG2 using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS14 antibody (67566-1-Ig, Clone: 3D5H5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RPS14 Monoclonal antibody, PBS Only

RPS14 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67566-1-PBS
CloneNo. 3D5H5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RPS 14, ribosomal protein S14, PRO2640, EMTB, 40S ribosomal protein S14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RPS14 Ready-To-Use IHC Kit. RPS14 primary antibody included.

产品信息

67566-1-PBS targets RPS14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RPS14 fusion protein Ag30032 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ribosomal protein S14
Synonyms RPS 14, ribosomal protein S14, PRO2640, EMTB, 40S ribosomal protein S14
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC003401
Gene Symbol RPS14
Gene ID (NCBI) 6208
RRIDAB_2882780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62263
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].