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RPS14 Monoclonal antibody

RPS14 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D5H5

Cat no : 67566-1-Ig

Print datasheet

Synonyms

40S ribosomal protein S14, EMTB, ribosomal protein S14, RPS14

验证数据展示

  • WB analysis using 67566-1-Ig

    WB analysis using 67566-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human urothelial carcinoma using 67566-1-Ig

    IHC staining of human urothelial carcinoma using 67566-1-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPS14 antibody included
  • IF Staining of HepG2 using 67566-1-Ig

    IF Staining of HepG2 using 67566-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67566-1-Ig (RPS14 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67566-1-Ig

    WB analysis using 67566-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, HuH-7 cells, K-562 cells, HSC-T6 cells, 4T1 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPS14 Ready-To-Use IHC Kit. RPS14 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67566-1-Ig targets RPS14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen RPS14 fusion protein Ag30032 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ribosomal protein S14
Synonyms 40S ribosomal protein S14, EMTB, ribosomal protein S14, RPS14
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC003401
Gene Symbol RPS14
Gene ID (NCBI) 6208
RRIDAB_2882780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].

实验方案

Product Specific Protocols
WB protocol for RPS14 antibody 67566-1-IgDownload protocol
IHC protocol for RPS14 antibody 67566-1-IgDownload protocol
IF protocol for RPS14 antibody 67566-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols