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GSS Monoclonal antibody

GSS Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig, hamster and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F12G1

Cat No : 67598-1-Ig

Print datasheet

Synonyms

Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase, GSHS, GSS

验证数据展示

  • WB analysis using 67598-1-Ig

    WB analysis using 67598-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 67598-1-Ig

    WB analysis of HEK-293 using 67598-1-Ig

    WB result of GSS antibody (67598-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells.

  • IF Staining of HeLa using 67598-1-Ig

    IF Staining of HeLa using 67598-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSS antibody (67598-1-Ig, Clone: 1F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human liver cancer using 67598-1-Ig

    IHC staining of human liver cancer using 67598-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSS antibody included
  • IHC staining of human liver cancer using 67598-1-Ig

    IHC staining of human liver cancer using 67598-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSS antibody included
  • WB analysis using 67598-1-Ig

    WB analysis using 67598-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inCHO cells, rat liver tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GSS Ready-To-Use IHC Kit. GSS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67598-1-Ig targets GSS in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, hamster samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat, pig, hamster
Cited Reactivityhuman
Immunogen GSS fusion protein Ag8354 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutathione synthetase
Synonyms Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase, GSHS, GSS
Calculated Molecular Weight 474 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007927
Gene Symbol GSS
Gene ID (NCBI) 2937
RRIDAB_2882804
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSS antibody 67598-1-IgDownload protocol
IHC protocol for GSS antibody 67598-1-IgDownload protocol
IF protocol for GSS antibody 67598-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

World J Gastroenterol

Bile acids inhibit ferroptosis sensitivity through activating farnesoid X receptor in gastric cancer cells

Authors - Chu-Xuan Liu
View Article
humanWB

J Biol Chem

Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity

Authors - Xiaohan Jin
View Article