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MYBPC3 Monoclonal antibody

MYBPC3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3C8F8

Cat no : 67608-1-Ig

Print datasheet

Synonyms

Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3

验证数据展示

  • IHC staining of human heart using 67608-1-Ig

    IHC staining of human heart using 67608-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse heart using 67608-1-Ig

    WB analysis of mouse heart using 67608-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat heart using 67608-1-Ig

    IF Staining of rat heart using 67608-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using MYBPC3 antibody (67608-1-Ig, Clone: 3C8F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inmouse heart tissue, H9C2 cells, rabbit heart tissue, pig heart tissue
Positive IHC detected inhuman heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat heart tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67608-1-Ig targets MYBPC3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen MYBPC3 fusion protein Ag15848 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin binding protein C, cardiac
Synonyms Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3
Calculated Molecular Weight 1274 aa, 141 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC151211
Gene Symbol MYBPC3
Gene ID (NCBI) 4607
RRIDAB_2882814
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14896
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).

实验方案

Product Specific Protocols
WB protocol for MYBPC3 antibody 67608-1-IgDownload protocol
IHC protocol for MYBPC3 antibody 67608-1-IgDownload protocol
IF protocol for MYBPC3 antibody 67608-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols