验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, mouse heart tissue |
For other applications, we recommend the unconjugated version of this antibody, 67608-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67608 targets MYBPC3 in WB applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human, mouse, rat, pig |
| 免疫原 | MYBPC3 fusion protein Ag15848 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | myosin binding protein C, cardiac |
| 别名 | Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3 |
| 计算分子量 | 1274 aa, 141 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC151211 |
| 基因名称 | MYBPC3 |
| Gene ID (NCBI) | 4607 |
| RRID | AB_3673521 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14896 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 MYBPC3 antibody CL594-67608 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |