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CoraLite®594-conjugated MYBPC3 Monoclonal antibody

MYBPC3 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C8F8

Cat No : CL594-67608

Print datasheet

Synonyms

Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3

验证数据展示

  • WB analysis using CL594-67608

    WB analysis using CL594-67608

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-67608 (MYBPC3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat heart tissue, mouse heart tissue
For other applications, we recommend the unconjugated version of this antibody, 67608-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67608 targets MYBPC3 in WB applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen MYBPC3 fusion protein Ag15848 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin binding protein C, cardiac
Synonyms Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3
Calculated Molecular Weight 1274 aa, 141 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC151211
Gene Symbol MYBPC3
Gene ID (NCBI) 4607
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14896
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).

实验方案

Product Specific Protocols
WB protocol for CL594 MYBPC3 antibody CL594-67608Download protocol
Standard Protocols
Click here to view our Standard Protocols