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验证数据展示

  • IHC staining of human heart using 67608-1-Ig

    IHC staining of human heart using 67608-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse heart using 67608-1-Ig

    WB analysis of mouse heart using 67608-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat heart using 67608-1-Ig

    IF Staining of rat heart using 67608-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using MYBPC3 antibody (67608-1-Ig, Clone: 3C8F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

MYBPC3 Monoclonal antibody

MYBPC3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67608-1-Ig

产品说明书

CloneNo. 3C8F8

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, ELISA

别名

Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, H9C2 cells, rabbit heart tissue, pig heart tissue
Positive IHC detected inhuman heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat heart tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67608-1-Ig targets MYBPC3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 MYBPC3 fusion protein Ag15848 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin binding protein C, cardiac
别名 Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3
计算分子量 1274 aa, 141 kDa
观测分子量 140-150 kDa
GenBank蛋白编号BC151211
基因名称 MYBPC3
Gene ID (NCBI) 4607
RRIDAB_2882814
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14896
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).

实验方案

Product Specific Protocols
WB protocol for MYBPC3 antibody 67608-1-IgDownload protocol
IHC protocol for MYBPC3 antibody 67608-1-IgDownload protocol
IF protocol for MYBPC3 antibody 67608-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols