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验证数据展示

  • WB analysis using 67616-1-Ig (same clone as 67616-1-PBS)

    WB analysis using 67616-1-Ig (same clone as 67616-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67616-1-Ig (NCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

    IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

    IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

    IF Staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCS1 antibody (67616-1-Ig, Clone: 2F10C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51282-1

    Cytometric bead array standard curve of MP51282-1

    Cytometric bead array standard curve of MP51282-1, FREQ Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67616-1-PBS. Detection antibody: 67616-2-PBS. Standard:Ag29808. Range: 1.563-100 ng/mL.

NCS1 Monoclonal antibody, PBS Only (Capture)

NCS1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67616-1-PBS

产品说明书

CloneNo. 2F10C8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67616-1-PBS targets NCS1 as part of a matched antibody pair:

MP51282-1: 67616-1-PBS capture and 67616-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 NCS1 fusion protein Ag29808 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 frequenin homolog (Drosophila)
别名 FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)
计算分子量 22 kDa
观测分子量 20 kDa
GenBank蛋白编号BC004856
基因名称 NCS1
Gene ID (NCBI) 23413
RRIDAB_2882819
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62166
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C