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PPID Monoclonal antibody

PPID Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2B1

Cat no : 67632-1-Ig

Print datasheet

Synonyms

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

验证数据展示

  • WB analysis using 67632-1-Ig

    WB analysis using 67632-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67632-1-Ig (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 67632-1-Ig

    IHC staining of human cervical cancer using 67632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67632-1-Ig (PPID antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPID antibody included
  • IHC staining of human cervical cancer using 67632-1-Ig

    IHC staining of human cervical cancer using 67632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67632-1-Ig (PPID antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPID antibody included

经过测试的应用

Positive WB detected inRaji cells, Jurkat cells, K-562 cells, JAR cells, pig brain issue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPID Ready-To-Use IHC Kit. PPID primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67632-1-Ig targets PPID in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PPID fusion protein Ag4371 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peptidylprolyl isomerase D
Synonyms Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase
Calculated Molecular Weight 370 aa, 41 kDa
Observed Molecular Weight 38-41 kDa
GenBank Accession NumberBC030707
Gene Symbol PPID
Gene ID (NCBI) 5481
RRIDAB_2882833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ08752
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

实验方案

Product Specific Protocols
WB protocol for PPID antibody 67632-1-IgDownload protocol
IHC protocol for PPID antibody 67632-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols