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验证数据展示

  • WB analysis using 67632-1-Ig (same clone as 67632-1-PBS)

    WB analysis using 67632-1-Ig (same clone as 67632-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67632-1-Ig (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67632-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 67632-1-Ig (same clone as 67632-1-PBS)

    IHC staining of human cervical cancer using 67632-1-Ig (same clone as 67632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67632-1-Ig (PPID antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67632-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPID antibody included
  • IHC staining of human cervical cancer using 67632-1-Ig (same clone as 67632-1-PBS)

    IHC staining of human cervical cancer using 67632-1-Ig (same clone as 67632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67632-1-Ig (PPID antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67632-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPID antibody included

PPID Monoclonal antibody, PBS Only

PPID Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67632-1-PBS

产品说明书

CloneNo. 1A2B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Indirect ELISA

别名

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PPID Ready-To-Use IHC Kit. PPID primary antibody included.

产品信息

67632-1-PBS targets PPID in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PPID fusion protein Ag4371 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidylprolyl isomerase D
别名 Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase
计算分子量 370 aa, 41 kDa
观测分子量 38-41 kDa
GenBank蛋白编号BC030707
基因名称 PPID
Gene ID (NCBI) 5481
RRIDAB_2882833
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ08752
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).