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验证数据展示

  • WB analysis using 67632-1-Ig

    WB analysis using 67632-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67632-1-Ig (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 67632-1-Ig

    IHC staining of human cervical cancer using 67632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67632-1-Ig (PPID antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPID antibody included
  • IHC staining of human cervical cancer using 67632-1-Ig

    IHC staining of human cervical cancer using 67632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67632-1-Ig (PPID antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPID antibody included

PPID Monoclonal antibody

PPID Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67632-1-Ig

产品说明书

CloneNo. 1A2B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, Jurkat cells, K-562 cells, JAR cells, pig brain issue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPID Ready-To-Use IHC Kit. PPID primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67632-1-Ig targets PPID in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PPID fusion protein Ag4371 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidylprolyl isomerase D
别名 Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase
计算分子量 370 aa, 41 kDa
观测分子量 38-41 kDa
GenBank蛋白编号BC030707
基因名称 PPID
Gene ID (NCBI) 5481
RRIDAB_2882833
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ08752
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

实验方案

Product Specific Protocols
WB protocol for PPID antibody 67632-1-IgDownload protocol
IHC protocol for PPID antibody 67632-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols