验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67649-1-Ig targets GNE in WB, ELISA applications and shows reactivity with Human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, rat |
| 文献引用反应性 | human |
| 免疫原 | GNE fusion protein Ag18702 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase |
| 别名 | DMRV, GLCNE, GNE, IBM2, ManAc kinase, NM, Uae1, UDP GlcNAc 2 epimerase |
| 计算分子量 | 722 aa, 79 kDa |
| 观测分子量 | 79 kDa |
| GenBank蛋白编号 | BC121179 |
| 基因名称 | GNE |
| Gene ID (NCBI) | 10020 |
| RRID | AB_2882849 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9Y223 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNE antibody 67649-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neurol Sci Clinical, genetic, and pathological characterization of GNE myopathy in China. | ||
Gene A novel mutation alters GNE bifunctional enzyme activity and leads to familial inherited GNE diseases |