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验证数据展示

  • WB analysis using 67680-1-Ig

    WB analysis using 67680-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 67680-1-Ig

    IF Staining of HeLa using 67680-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXT1 antibody (67680-1-Ig, Clone: 2C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67680-1-Ig

    WB analysis using 67680-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

NXT1 Monoclonal antibody

NXT1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67680-1-Ig

产品说明书

CloneNo. 2C10A2
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat and More (1)

应用

WB, IF/ICC, ELISA

别名

MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67680-1-Ig targets NXT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat
文献引用反应性human
免疫原 NXT1 fusion protein Ag30039 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 NTF2-like export factor 1
别名 MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15
计算分子量 15 kDa
观测分子量15 kDa
GenBank蛋白编号BC000759
基因名称 NXT1
Gene ID (NCBI) 29107
RRIDAB_2882873
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UKK6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NXT1 is located in the nuclear envelope and is homologous to nuclear transport factor 2. NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. NXT1 also heterodimerizes with Tap protein and regulates the ability of Tap protein to mediate nuclear mRNA export.

实验方案

Product Specific Protocols
WB protocol for NXT1 antibody 67680-1-IgDownload protocol
IF protocol for NXT1 antibody 67680-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
View Article