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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

    WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67680-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67680-1-Ig (same clone as 67680-1-PBS)

    IF Staining of HeLa using 67680-1-Ig (same clone as 67680-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXT1 antibody (67680-1-Ig, Clone: 2C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67680-1-PBS in a different storage buffer formulation.

  • WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

    WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67680-1-PBS in a different storage buffer formulation.

NXT1 Monoclonal antibody, PBS Only

NXT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67680-1-PBS

产品说明书

CloneNo. 2C10A2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67680-1-PBS targets NXT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 NXT1 fusion protein Ag30039 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 NTF2-like export factor 1
别名 MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15
计算分子量 15 kDa
观测分子量15 kDa
GenBank蛋白编号BC000759
基因名称 NXT1
Gene ID (NCBI) 29107
RRIDAB_2882873
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UKK6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NXT1 is located in the nuclear envelope and is homologous to nuclear transport factor 2. NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. NXT1 also heterodimerizes with Tap protein and regulates the ability of Tap protein to mediate nuclear mRNA export.