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NXT1 Monoclonal antibody, PBS Only

NXT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C10A2

Cat no : 67680-1-PBS

Print datasheet

Synonyms

MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15

验证数据展示

  • WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

    WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67680-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67680-1-Ig (same clone as 67680-1-PBS)

    IF Staining of HeLa using 67680-1-Ig (same clone as 67680-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXT1 antibody (67680-1-Ig, Clone: 2C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67680-1-PBS in a different storage buffer formulation.

  • WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

    WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67680-1-PBS in a different storage buffer formulation.

产品信息

67680-1-PBS targets NXT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NXT1 fusion protein Ag30039 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NTF2-like export factor 1
Synonyms MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15
Calculated Molecular Weight 15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC000759
Gene Symbol NXT1
Gene ID (NCBI) 29107
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKK6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NXT1 is located in the nuclear envelope and is homologous to nuclear transport factor 2. NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. NXT1 also heterodimerizes with Tap protein and regulates the ability of Tap protein to mediate nuclear mRNA export.