Laminin beta 1 Monoclonal antibody
Laminin beta 1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B8E9
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, NIH/3T3 cells, human placenta tissue, U2OS cells, A549 cells, HeLa cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
67705-1-Ig targets Laminin beta 1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, Mouse |
Cited Reactivity | human |
Immunogen | Laminin beta 1 fusion protein Ag19182 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | laminin, beta 1 |
Synonyms | CLM, LAMB1, Laminin 1 subunit beta, Laminin 10 subunit beta, Laminin 12 subunit beta, Laminin 2 subunit beta, Laminin 6 subunit beta, Laminin 8 subunit beta, Laminin B1 chain, Laminin beta 1, Laminin subunit beta 1, laminin, beta 1 |
Calculated Molecular Weight | 1786 aa, 198 kDa |
Observed Molecular Weight | 220-240 kDa |
GenBank Accession Number | BC113455 |
Gene Symbol | Laminin beta 1 |
Gene ID (NCBI) | 3912 |
RRID | AB_2882896 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P07942 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Laminins are a family of basement membrane glycoproteins composed of three nonidentical chains, laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) (PMID: 7959779). Laminins contribute to the structure of extracellular matrix and influence the behavior of associated cells, such as adhesion, differentiation, migration, phenotype stability, and resistance to anoikis (PMID: 23057746). Laminin beta 1 (LAMB1) is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin-1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. Laminin beta 1 is also a subunit of laminin-2 (laminin-211 or merosin), laminin-6 (laminin-311 or K-laminin), laminin-8 (laminin-411), laminin-10 (laminin-511) and laminin-12 (laminin-213).
实验方案
Product Specific Protocols | |
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WB protocol for Laminin beta 1 antibody 67705-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Diabet Med Mouse islet-derived stellate cells are similar to, but distinct from, mesenchymal stromal cells and influence the beta cell function |