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验证数据展示

  • WB analysis using 67712-1-Ig

    WB analysis using 67712-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 67712-1-Ig

    IHC staining of human lung cancer using 67712-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPRS antibody included
  • IF Staining of HeLa using 67712-1-Ig

    IF Staining of HeLa using 67712-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 67712-1-Ig

    IF Staining of HeLa using 67712-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • IP experiment of HeLa using 67712-1-Ig

    IP experiment of HeLa using 67712-1-Ig

    IP result of anti-EPRS (IP:67712-1-Ig, 4ug; Detection:67712-1-Ig 1:10000) with HeLa cells lysate 1085 ug.

  • IF Staining of BV-2 using 67712-1-Ig

    IF Staining of BV-2 using 67712-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed BV-2 cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

EPRS Monoclonal antibody

EPRS Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 67712-1-Ig

产品说明书

CloneNo. 1B7G2
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

EPRS1, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, BV-2 cells
Planning an IHC experiment? We recommend our IHCeasy EPRS Ready-To-Use IHC Kit. EPRS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67712-1-Ig targets EPRS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 EPRS fusion protein Ag19184 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamyl-prolyl-tRNA synthetase
别名 EPRS1, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase
计算分子量 1512 aa, 171 kDa
观测分子量171 kDa
GenBank蛋白编号BC126275
基因名称 EPRS
Gene ID (NCBI) 2058
RRIDAB_2882902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07814
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)

实验方案

Product Specific Protocols
WB protocol for EPRS antibody 67712-1-IgDownload protocol
IHC protocol for EPRS antibody 67712-1-IgDownload protocol
IF protocol for EPRS antibody 67712-1-IgDownload protocol
IP protocol for EPRS antibody 67712-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF,IHC

Kaohsiung J Med Sci

Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells

Authors - Li-Qi Lin
View Article