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EPRS Monoclonal antibody, PBS Only

EPRS Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7G2

Cat no : 67712-1-PBS

Print datasheet

Synonyms

DKFZp313B047, EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS

验证数据展示

  • WB analysis using 67712-1-Ig (same clone as 67712-1-PBS)

    WB analysis using 67712-1-Ig (same clone as 67712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67712-1-Ig (same clone as 67712-1-PBS)

    IHC staining of human lung cancer using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPRS antibody included
  • IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IP experiment of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IP result of anti-EPRS (IP:67712-1-Ig, 4ug; Detection:67712-1-Ig 1:10000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EPRS Ready-To-Use IHC Kit. EPRS primary antibody included.

产品信息

67712-1-PBS targets EPRS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen EPRS fusion protein Ag19184 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glutamyl-prolyl-tRNA synthetase
Synonyms DKFZp313B047, EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS
Calculated Molecular Weight 1512 aa, 171 kDa
Observed Molecular Weight171 kDa
GenBank Accession NumberBC126275
Gene Symbol EPRS
Gene ID (NCBI) 2058
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07814
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)