EPRS Monoclonal antibody
EPRS Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B7G2
验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, BV-2 cells |
Planning an IHC experiment? We recommend our IHCeasy EPRS Ready-To-Use IHC Kit. EPRS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67712-1-Ig targets EPRS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | EPRS fusion protein Ag19184 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glutamyl-prolyl-tRNA synthetase |
| Synonyms | EPRS1, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase |
| Calculated Molecular Weight | 1512 aa, 171 kDa |
| Observed Molecular Weight | 171 kDa |
| GenBank Accession Number | BC126275 |
| Gene Symbol | EPRS |
| Gene ID (NCBI) | 2058 |
| RRID | AB_2882902 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07814 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPRS antibody 67712-1-Ig | Download protocol |
| IHC protocol for EPRS antibody 67712-1-Ig | Download protocol |
| IF protocol for EPRS antibody 67712-1-Ig | Download protocol |
| IP protocol for EPRS antibody 67712-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
