验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, rabbit cerebellum tissue, rat brain tissue, mouse brain tissue, chicken brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
67717-1-Ig targets NMDAR1/GRIN1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat, rabbit, chicken |
| 文献引用反应性 | mouse, rat |
| 免疫原 | NMDAR1/GRIN1 fusion protein Ag26364 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glutamate receptor, ionotropic, N-methyl D-aspartate 1 |
| 别名 | GRIN1, NMDAR1, NMDAR 1, Glutamate receptor ionotropic, NMDA 1, GluN1 |
| 计算分子量 | 105 kDa |
| 观测分子量 | 105-120 kDa |
| GenBank蛋白编号 | NM_000832 |
| 基因名称 | NMDAR1 |
| Gene ID (NCBI) | 2902 |
| RRID | AB_2882906 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NMDAR1/GRIN1 antibody 67717-1-Ig | Download protocol |
| IHC protocol for NMDAR1/GRIN1 antibody 67717-1-Ig | Download protocol |
| IF protocol for NMDAR1/GRIN1 antibody 67717-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Brain Res The NR2B-targeted intervention alleviates the neuronal injuries at the sub-acute stage of cerebral ischemia: an exploration of stage-dependent strategy against ischemic insults | ||
Heliyon A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice | ||
Biomedicines D-Serine May Ameliorate Hippocampal Synaptic Plasticity Impairment Induced by Patients' Anti-N-methyl-D-aspartate Receptor Antibodies in Mice | ||
Biomedicines Impaired Response to Mismatch Novelty in the Li2+-Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs |
