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验证数据展示

  • WB analysis using 67723-1-Ig

    WB analysis using 67723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67723-1-Ig

    WB analysis using 67723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67723-1-Ig

    WB analysis using 67723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 67723-1-Ig

    WB analysis of U2OS using 67723-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67723-1-Ig

    WB analysis of NIH/3T3 using 67723-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 67723-1-Ig

    IF Staining of U2OS using 67723-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSIP1 antibody (67723-1-Ig, Clone: 1E9E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

PSIP1 Monoclonal antibody

PSIP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67723-1-Ig

产品说明书

CloneNo. 1E9E7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, U2OS cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PSIP1 Ready-To-Use IHC Kit. PSIP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67723-1-Ig targets PSIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PSIP1 fusion protein Ag30562 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PC4 and SFRS1 interacting protein 1
别名 Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7
计算分子量 60 kDa
观测分子量75 kDa
GenBank蛋白编号NM_021144
基因名称 PSIP1
Gene ID (NCBI) 11168
RRIDAB_3670379
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75475
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.

实验方案

Product Specific Protocols
WB protocol for PSIP1 antibody 67723-1-IgDownload protocol
IF protocol for PSIP1 antibody 67723-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols