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PSIP1 Monoclonal antibody

PSIP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E9E7

Cat no : 67723-1-Ig

Print datasheet

Synonyms

Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7

验证数据展示

  • WB analysis using 67723-1-Ig

    WB analysis using 67723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67723-1-Ig

    WB analysis using 67723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67723-1-Ig

    WB analysis using 67723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 67723-1-Ig

    WB analysis of U2OS using 67723-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67723-1-Ig

    WB analysis of NIH/3T3 using 67723-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 67723-1-Ig

    IF Staining of U2OS using 67723-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSIP1 antibody (67723-1-Ig, Clone: 1E9E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, U2OS cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PSIP1 Ready-To-Use IHC Kit. PSIP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67723-1-Ig targets PSIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PSIP1 fusion protein Ag30562 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name PC4 and SFRS1 interacting protein 1
Synonyms Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7
Calculated Molecular Weight 60 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberNM_021144
Gene Symbol PSIP1
Gene ID (NCBI) 11168
RRIDAB_3670379
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75475
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSIP1 enables DNA-binding transcription factor binding activity, chromatin binding activity and transcription coactivator activity. It is involved in mRNA 5'-splice site recognition and positive regulation of transcription by RNA polymerase II.

实验方案

Product Specific Protocols
WB protocol for PSIP1 antibody 67723-1-IgDownload protocol
IF protocol for PSIP1 antibody 67723-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols