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验证数据展示

  • WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

    WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

  • WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

    WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

  • WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

    WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 67723-1-Ig (same clone as 67723-1-PBS)

    WB analysis of U2OS using 67723-1-Ig (same clone as 67723-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67723-1-Ig (same clone as 67723-1-PBS)

    WB analysis of NIH/3T3 using 67723-1-Ig (same clone as 67723-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67723-1-Ig (same clone as 67723-1-PBS)

    IF Staining of U2OS using 67723-1-Ig (same clone as 67723-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSIP1 antibody (67723-1-Ig, Clone: 1E9E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50375-1

    Cytometric bead array standard curve of MP50375-1

    Cytometric bead array standard curve of MP50375-1, PSIP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67723-1-PBS. Detection antibody: 68941-1-PBS. Standard:Ag21886. Range: 3.125-200 ng/mL.

PSIP1 Monoclonal antibody, PBS Only (Capture)

PSIP1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67723-1-PBS

产品说明书

CloneNo. 1E9E7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSIP1 Ready-To-Use IHC Kit. PSIP1 primary antibody included.

产品信息

67723-1-PBS targets PSIP1 as part of a matched antibody pair:

MP50375-1: 67723-1-PBS capture and 68941-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PSIP1 fusion protein Ag30562 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PC4 and SFRS1 interacting protein 1
别名 Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7
计算分子量 60 kDa
观测分子量75 kDa
GenBank蛋白编号NM_021144
基因名称 PSIP1
Gene ID (NCBI) 11168
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75475
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.