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验证数据展示

  • WB analysis using 67723-1-Ig

    WB analysis using 67723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67723-1-Ig

    WB analysis using 67723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67723-1-Ig

    WB analysis using 67723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 67723-1-Ig

    WB analysis of U2OS using 67723-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67723-1-Ig

    WB analysis of NIH/3T3 using 67723-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 67723-1-Ig

    IF Staining of U2OS using 67723-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSIP1 antibody (67723-1-Ig, Clone: 1E9E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

PSIP1 Monoclonal antibody

PSIP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67723-1-Ig

Print datasheet

CloneNo. 1E9E7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, U2OS cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PSIP1 Ready-To-Use IHC Kit. PSIP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67723-1-Ig targets PSIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PSIP1 fusion protein Ag30562 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name PC4 and SFRS1 interacting protein 1
Synonyms Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7
Calculated Molecular Weight 60 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberNM_021144
Gene Symbol PSIP1
Gene ID (NCBI) 11168
RRIDAB_3670379
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75475
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.

实验方案

Product Specific Protocols
WB protocol for PSIP1 antibody 67723-1-IgDownload protocol
IF protocol for PSIP1 antibody 67723-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols