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WWOX Monoclonal antibody

WWOX Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8F4

Cat no : 67725-1-Ig

Print datasheet

Synonyms

D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX

验证数据展示

  • WB analysis using 67725-1-Ig

    WB analysis using 67725-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67725-1-Ig (WWOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 67725-1-Ig

    IHC staining of mouse testis using 67725-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67725-1-Ig (WWOX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WWOX antibody included

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, pig liver tissue, rat liver tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy WWOX Ready-To-Use IHC Kit. WWOX primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67725-1-Ig targets WWOX in WB, IHC, ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, rat, pig, mouse
Immunogen WWOX fusion protein Ag7969 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name WW domain containing oxidoreductase
Synonyms D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX
Calculated Molecular Weight 47 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC003184
Gene Symbol WWOX
Gene ID (NCBI) 51741
RRIDAB_2882911
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for WWOX antibody 67725-1-IgDownload protocol
IHC protocol for WWOX antibody 67725-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols