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验证数据展示

  • WB analysis using 67745-1-Ig

    WB analysis using 67745-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67745-1-Ig (SKP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 67745-1-Ig

    IF Staining of MCF-7 using 67745-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SKP1 antibody (67745-1-Ig, Clone: 1D5G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SKP1 Monoclonal antibody

SKP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67745-1-Ig

产品说明书

CloneNo. 1D5G4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

EMC19, OCP 2, OCP II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, SIII, SKP1, SKP1A, TCEB1L

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SKP1 Ready-To-Use IHC Kit. SKP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67745-1-Ig targets SKP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 SKP1 fusion protein Ag30709 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 S-phase kinase-associated protein 1
别名 EMC19, OCP 2, OCP II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, SIII, SKP1, SKP1A, TCEB1L
计算分子量 19 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC009839
基因名称 SKP1
Gene ID (NCBI) 6500
RRIDAB_2918514
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP63208
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.

实验方案

Product Specific Protocols
WB protocol for SKP1 antibody 67745-1-IgDownload protocol
IF protocol for SKP1 antibody 67745-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols