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SKP1 Monoclonal antibody, PBS Only

SKP1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5G4

Cat no : 67745-1-PBS

Print datasheet

Synonyms

EMC19, OCP 2, OCP II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, SIII, SKP1, SKP1A, TCEB1L

验证数据展示

  • WB analysis using 67745-1-Ig (same clone as 67745-1-PBS)

    WB analysis using 67745-1-Ig (same clone as 67745-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67745-1-Ig (SKP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67745-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67745-1-Ig (same clone as 67745-1-PBS)

    IF Staining of MCF-7 using 67745-1-Ig (same clone as 67745-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SKP1 antibody (67745-1-Ig, Clone: 1D5G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67745-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SKP1 Ready-To-Use IHC Kit. SKP1 primary antibody included.

产品信息

67745-1-PBS targets SKP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SKP1 fusion protein Ag30709 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name S-phase kinase-associated protein 1
Synonyms EMC19, OCP 2, OCP II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, SIII, SKP1, SKP1A, TCEB1L
Calculated Molecular Weight 19 kDa
Observed Molecular Weight18-20 kDa
GenBank Accession NumberBC009839
Gene Symbol SKP1
Gene ID (NCBI) 6500
RRIDAB_2918514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63208
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.