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ITCH Monoclonal antibody

ITCH Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

2E3G9

Cat no : 67757-1-Ig

Print datasheet

Synonyms

AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1

验证数据展示

  • WB analysis using 67757-1-Ig

    WB analysis using 67757-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67757-1-Ig

    IF Staining of HepG2 using 67757-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ITCH antibody (67757-1-Ig, Clone: 2E3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, K-562 cells, LNCaP cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ITCH Ready-To-Use IHC Kit. ITCH primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67757-1-Ig targets ITCH in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen ITCH fusion protein Ag14688 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name itchy E3 ubiquitin protein ligase homolog (mouse)
Synonyms AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1
Calculated Molecular Weight 903 aa, 103 kDa
Observed Molecular Weight103 kDa, 70 kDa
GenBank Accession NumberBC011571
Gene Symbol ITCH
Gene ID (NCBI) 83737
RRIDAB_2918525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96J02
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ITCH antibody 67757-1-IgDownload protocol
IF protocol for ITCH antibody 67757-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Biomedicines

ITCH-Mediated Ubiquitylation of ITGB3 Promotes Cell Proliferation and Invasion of Ectopic Endometrial Stromal Cells in Ovarian Endometriosis

Authors - Liansuo Zhang
View Article
humanIF

Sci Rep

Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinoma

Authors - Zehua Lin
View Article