ITCH Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E3G9

Cat No : 67757-1-PBS

Synonyms

AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1

WB analysis using 67757-1-Ig (same clone as 67757-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67757-1-Ig (same clone as 67757-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ITCH antibody (67757-1-Ig, Clone: 2E3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

Size

Planning an IHC experiment? We recommend our IHCeasy ITCH Ready-To-Use IHC Kit. ITCH primary antibody included.

67757-1-PBS targets ITCH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications	WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity	Human, mouse, rat
Immunogen	ITCH fusion protein Ag14688 种属同源性预测
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Full Name	itchy E3 ubiquitin protein ligase homolog (mouse)
Synonyms	AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1
Calculated Molecular Weight	903 aa, 103 kDa
Observed Molecular Weight	103 kDa, 70 kDa
GenBank Accession Number	BC011571
Gene Symbol	ITCH
Gene ID (NCBI)	83737
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q96J02
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C