ITCH Monoclonal antibody

ITCH Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67757-1-Ig

产品说明书

CloneNo. 2E3G9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat and More (1)

应用

WB, IF/ICC, ELISA and More (2)

AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, K-562 cells, LNCaP cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ITCH Ready-To-Use IHC Kit. ITCH primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67757-1-Ig targets ITCH in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 Human, mouse, rat
文献引用反应性human
免疫原 ITCH fusion protein Ag14688 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 itchy E3 ubiquitin protein ligase homolog (mouse)
别名 AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1
计算分子量 903 aa, 103 kDa
观测分子量103 kDa, 70 kDa
GenBank蛋白编号BC011571
基因名称 ITCH
Gene ID (NCBI) 83737
RRIDAB_2918525
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96J02
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ITCH antibody 67757-1-IgDownload protocol
IF protocol for ITCH antibody 67757-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

EMBO J

A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease

Authors - Kyungtae Lim
humanWB,IP

Biomedicines

ITCH-Mediated Ubiquitylation of ITGB3 Promotes Cell Proliferation and Invasion of Ectopic Endometrial Stromal Cells in Ovarian Endometriosis

Authors - Liansuo Zhang
humanIF

Sci Rep

Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinoma

Authors - Zehua Lin
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