验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HSC-T6 cells, K-562 cells, LNCaP cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67757-1-Ig targets ITCH in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF, IP |
经测试反应性 | Human, mouse, rat |
文献引用反应性 | human |
免疫原 | ITCH fusion protein Ag14688 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | itchy E3 ubiquitin protein ligase homolog (mouse) |
别名 | AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1 |
计算分子量 | 903 aa, 103 kDa |
观测分子量 | 103 kDa, 70 kDa |
GenBank蛋白编号 | BC011571 |
基因名称 | ITCH |
Gene ID (NCBI) | 83737 |
RRID | AB_2918525 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q96J02 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for ITCH antibody 67757-1-Ig | Download protocol |
IF protocol for ITCH antibody 67757-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
EMBO J A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease | ||
Biomedicines ITCH-Mediated Ubiquitylation of ITGB3 Promotes Cell Proliferation and Invasion of Ectopic Endometrial Stromal Cells in Ovarian Endometriosis | ||
Sci Rep Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinoma |