gp130/IL6ST Monoclonal antibody
gp130/IL6ST Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
1H7C5
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, NCCIT cells, PC-3 cells, DU 145 cells |
| Positive IHC detected in | human placenta tissue, human colon cancer tissue, human liver cancer tissue, human lung cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy IL6ST/GP130 Ready-To-Use IHC Kit. IL6ST/GP130 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67766-1-Ig targets gp130/IL6ST in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | gp130/IL6ST fusion protein Ag28808 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 6 signal transducer (gp130, oncostatin M receptor) |
| Synonyms | IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130 |
| Calculated Molecular Weight | 104 kDa |
| Observed Molecular Weight | 130-140 kDa |
| GenBank Accession Number | NM_002184 |
| Gene Symbol | IL6ST |
| Gene ID (NCBI) | 3572 |
| RRID | AB_2918533 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P40189 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for gp130/IL6ST antibody 67766-1-Ig | Download protocol |
| IHC protocol for gp130/IL6ST antibody 67766-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Prolif Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling. | ||
Cancer Med Construction of a prognostic model for triple-negative breast cancer based on immune-related genes, and associations between the tumor immune microenvironment and immunological therapy |
