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gp130/IL6ST Monoclonal antibody, PBS Only

gp130/IL6ST Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H7C5

Cat no : 67766-1-PBS

Print datasheet

Synonyms

IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130

验证数据展示

  • WB analysis using 67766-1-Ig (same clone as 67766-1-PBS)

    WB analysis using 67766-1-Ig (same clone as 67766-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67766-1-Ig (gp130 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)

    IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IL6ST/GP130 antibody included
  • IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)

    IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IL6ST/GP130 antibody included
Planning an IHC experiment? We recommend our IHCeasy IL6ST/GP130 Ready-To-Use IHC Kit. IL6ST/GP130 primary antibody included.

产品信息

67766-1-PBS targets gp130/IL6ST in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen gp130/IL6ST fusion protein Ag28808 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 6 signal transducer (gp130, oncostatin M receptor)
Synonyms IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130
Calculated Molecular Weight 104 kDa
Observed Molecular Weight130-140 kDa
GenBank Accession NumberNM_002184
Gene Symbol IL6ST
Gene ID (NCBI) 3572
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40189
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).