IFI16 Monoclonal antibody
IFI16 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1C10D7
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, MOLT-4 cells, Ramos cells, Daudi cells |
| Positive IHC detected in | human tonsillitis tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy IFI16 Ready-To-Use IHC Kit. IFI16 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
67790-1-Ig targets IFI16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | IFI16 fusion protein Ag30314 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interferon, gamma-inducible protein 16 |
| Synonyms | Ifi 16, IFI16, IFNGIP1, PYHIN2 |
| Calculated Molecular Weight | 729 aa, 82 kDa |
| Observed Molecular Weight | 85-95 kDa |
| GenBank Accession Number | BC017059 |
| Gene Symbol | IFI16 |
| Gene ID (NCBI) | 3428 |
| RRID | AB_2918554 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16666 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFI16 antibody 67790-1-Ig | Download protocol |
| IHC protocol for IFI16 antibody 67790-1-Ig | Download protocol |
| IF protocol for IFI16 antibody 67790-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Transl Med IFI16 promotes the progression of clear cell renal cell carcinoma through the IL6/PI3K/AKT axis |
