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验证数据展示

  • WB analysis using 67796-1-Ig

    WB analysis using 67796-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67796-1-Ig (TWF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of MCF-7 using 67796-1-Ig

    IF Staining of MCF-7 using 67796-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TWF1 antibody (67796-1-Ig, Clone: 2F10F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

TWF1 Monoclonal antibody

TWF1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67796-1-Ig

产品说明书

CloneNo. 2F10F11

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

A6, Protein A6, Protein tyrosine kinase 9, PTK9, TWF1, Twinfilin 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy TWF1 Ready-To-Use IHC Kit. TWF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67796-1-Ig targets TWF1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 TWF1 fusion protein Ag28630 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 twinfilin, actin-binding protein, homolog 1 (Drosophila)
别名 A6, Protein A6, Protein tyrosine kinase 9, PTK9, TWF1, Twinfilin 1
计算分子量 368 aa, 42 kDa
观测分子量36-38 kDa
GenBank蛋白编号BC022344
基因名称 TWF1
Gene ID (NCBI) 5756
RRIDAB_2918560
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12792
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Twinfilin-1 (TWF1) is an actin-binding protein implicated in cell motility, invasion and migration. Its expression is linked to sensitivity to drugs and cancer progression. Overexpression of TWF1 has been reported in various cancers, including breast cancer and lung cancer.

实验方案

Product Specific Protocols
WB protocol for TWF1 antibody 67796-1-IgDownload protocol
IF protocol for TWF1 antibody 67796-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols