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MFG-E8 Monoclonal antibody

MFG-E8 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A9

Cat no : 67797-1-Ig

Print datasheet

Synonyms

BA46, Breast epithelial antigen BA46, EDIL1, HMFG, hP47, HsT19888, Lactadherin, MFG E8, MFGE8, MFG-E8, MFGM, Milk fat globule EGF factor 8, OAcGD3S, SED1, SPAG10

验证数据展示

  • WB analysis of human milk using 67797-1-Ig

    WB analysis of human milk using 67797-1-Ig

    human milk were subjected to SDS PAGE followed by western blot with 67797-1-Ig (MFG-E8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 67797-1-Ig

    IHC staining of human placenta using 67797-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67797-1-Ig (MFG-E8 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 67797-1-Ig

    IHC staining of human placenta using 67797-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67797-1-Ig (MFG-E8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman milk, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67797-1-Ig targets MFG-E8 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen MFG-E8 fusion protein Ag30666 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name milk fat globule-EGF factor 8 protein
Synonyms BA46, Breast epithelial antigen BA46, EDIL1, HMFG, hP47, HsT19888, Lactadherin, MFG E8, MFGE8, MFG-E8, MFGM, Milk fat globule EGF factor 8, OAcGD3S, SED1, SPAG10
Calculated Molecular Weight 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC003610
Gene Symbol MFGE8
Gene ID (NCBI) 4240
RRIDAB_2918561
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), also known as lactadherin, is a multifunctional secreted glycoprotein exhibiting versatile functions in cell physiology affecting health and diseases. It plays roles in clearance of apoptotic cells, anti-inflammatory, wound healing, arterial remodeling, angiogenesis, enhancing tumorigenicity and cancer metastasis (PMID: 23972256). MFG-E8 consists of two-repeated EGF-likedomains, a mucin-like domain and two-repeated discoidin-like domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain (PMID: 11856354). It shows ubiquitous pattern of expression in various types of cells and tissues. Altered expression of MFG-E8 causes disruptions of homeostasis and is correlated with a number of diseases (PMID: 27429513).

实验方案

Product Specific Protocols
WB protocol for MFG-E8 antibody 67797-1-IgDownload protocol
IHC protocol for MFG-E8 antibody 67797-1-IgDownload protocol
FC protocol for MFG-E8 antibody 67797-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols