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MFG-E8 Monoclonal antibody, PBS Only

MFG-E8 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1A9

Cat no : 67797-1-PBS

Print datasheet

Synonyms

BA46, Breast epithelial antigen BA46, EDIL1, HMFG, hP47, HsT19888, Lactadherin, MFG E8, MFGE8, MFG-E8, MFGM, Milk fat globule EGF factor 8, OAcGD3S, SED1, SPAG10

验证数据展示

  • WB analysis of human milk using 67797-1-Ig (same clone as 67797-1-PBS)

    WB analysis of human milk using 67797-1-Ig (same clone as 67797-1-PBS)

    human milk were subjected to SDS PAGE followed by western blot with 67797-1-Ig (MFG-E8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67797-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 67797-1-Ig (same clone as 67797-1-PBS)

    IHC staining of human placenta using 67797-1-Ig (same clone as 67797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67797-1-Ig (MFG-E8 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67797-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 67797-1-Ig (same clone as 67797-1-PBS)

    IHC staining of human placenta using 67797-1-Ig (same clone as 67797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67797-1-Ig (MFG-E8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67797-1-PBS in a different storage buffer formulation.

产品信息

67797-1-PBS targets MFG-E8 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen MFG-E8 fusion protein Ag30666 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name milk fat globule-EGF factor 8 protein
Synonyms BA46, Breast epithelial antigen BA46, EDIL1, HMFG, hP47, HsT19888, Lactadherin, MFG E8, MFGE8, MFG-E8, MFGM, Milk fat globule EGF factor 8, OAcGD3S, SED1, SPAG10
Calculated Molecular Weight 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC003610
Gene Symbol MFGE8
Gene ID (NCBI) 4240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08431
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), also known as lactadherin, is a multifunctional secreted glycoprotein exhibiting versatile functions in cell physiology affecting health and diseases. It plays roles in clearance of apoptotic cells, anti-inflammatory, wound healing, arterial remodeling, angiogenesis, enhancing tumorigenicity and cancer metastasis (PMID: 23972256). MFG-E8 consists of two-repeated EGF-likedomains, a mucin-like domain and two-repeated discoidin-like domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain (PMID: 11856354). It shows ubiquitous pattern of expression in various types of cells and tissues. Altered expression of MFG-E8 causes disruptions of homeostasis and is correlated with a number of diseases (PMID: 27429513).