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验证数据展示

  • WB analysis of A375 using 67838-1-Ig

    WB analysis of A375 using 67838-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67838-1-Ig

    IF Staining of A431 using 67838-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CAPNS1 antibody (67838-1-Ig, Clone: 2B6G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CAPNS1 Monoclonal antibody

CAPNS1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67838-1-Ig

产品说明书

CloneNo. 2B6G6

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

WB, IF/ICC, ELISA

别名

30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, A431 cells, HeLa cells, K-562 cells, THP-1 cells, HSC-T6 cells, PC-12 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CAPNS1 Ready-To-Use IHC Kit. CAPNS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67838-1-Ig targets CAPNS1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, rat
免疫原 CAPNS1 fusion protein Ag21885 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calpain, small subunit 1
别名 30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1
计算分子量 28 kDa
GenBank蛋白编号BC011903
基因名称 CAPNS1
Gene ID (NCBI) 826
RRIDAB_2918600
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04632
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPNS1 antibody 67838-1-IgDownload protocol
IF protocol for CAPNS1 antibody 67838-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols