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验证数据展示

  • IF Staining of A431 using CL488-67838

    IF Staining of A431 using CL488-67838

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 CAPNS1 antibody (CL488-67838, Clone: 2B6G6 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67838

    FC experiment of HeLa using CL488-67838

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CAPNS1 (CL488-67838, Clone:2B6G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated CAPNS1 Monoclonal antibody

CAPNS1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67838

产品说明书

CloneNo. 2B6G6

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

IF/ICC, FC (Intra)

别名

CAPN4, CAPNS, Calpain small subunit 1, Calpain regulatory subunit, Calcium-dependent protease small subunit 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CAPNS1 Ready-To-Use IHC Kit. CAPNS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67838-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67838 targets CAPNS1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat
免疫原 CAPNS1 fusion protein Ag21885 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calpain, small subunit 1
别名 CAPN4, CAPNS, Calpain small subunit 1, Calpain regulatory subunit, Calcium-dependent protease small subunit 1
计算分子量 28 kDa
GenBank蛋白编号BC011903
基因名称 CAPNS1
Gene ID (NCBI) 826
RRIDAB_2919558
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04632
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPNS1 is also named as CANP, CDPS, CSS1, CANPS, CAPN4 and CALPAIN4. It is a member of the calpain small subunit family, and belongs to the calcium-dependent family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. The small regulatory subunit consists of an N-terminal domain, containing about 30% glycine residues and a C-terminal Ca-binding domain (PMID: 9228946). This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CAPNS1 antibody CL488-67838Download protocol
Standard Protocols
Click here to view our Standard Protocols