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CAPNS1 Monoclonal antibody

CAPNS1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B6G6

Cat no : 67838-1-Ig

Print datasheet

Synonyms

30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1

验证数据展示

  • WB analysis of A375 using 67838-1-Ig

    WB analysis of A375 using 67838-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67838-1-Ig

    IF Staining of A431 using 67838-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CAPNS1 antibody (67838-1-Ig, Clone: 2B6G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, A431 cells, HeLa cells, K-562 cells, THP-1 cells, HSC-T6 cells, PC-12 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CAPNS1 Ready-To-Use IHC Kit. CAPNS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67838-1-Ig targets CAPNS1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, rat
Immunogen CAPNS1 fusion protein Ag21885 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calpain, small subunit 1
Synonyms 30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC011903
Gene Symbol CAPNS1
Gene ID (NCBI) 826
RRIDAB_2918600
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04632
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPNS1 antibody 67838-1-IgDownload protocol
IF protocol for CAPNS1 antibody 67838-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols