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验证数据展示

  • WB analysis using 67839-1-Ig

    WB analysis using 67839-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67839-1-Ig (MED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67839-1-Ig

    IHC staining of human breast cancer using 67839-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67839-1-Ig (MED4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67839-1-Ig

    IF Staining of HepG2 using 67839-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED4 antibody (67839-1-Ig, Clone: 2E6F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue).

MED4 Monoclonal antibody

MED4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67839-1-Ig

产品说明书

CloneNo. 2E6F4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ARC36, DRIP36, FLJ10956, HSPC126, MED4, mediator complex subunit 4, RP11 90M2.2, TRAP36, VDRIP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Hela, HEK-293, Jurkat, K-562, HSC-T6, PC-12, NIH/3T3, 4T1
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67839-1-Ig targets MED4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, rat
免疫原 MED4 fusion protein Ag8562 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mediator complex subunit 4
别名 ARC36, DRIP36, FLJ10956, HSPC126, MED4, mediator complex subunit 4, RP11 90M2.2, TRAP36, VDRIP
计算分子量 270 aa, 30 kDa
观测分子量~36 kDa
GenBank蛋白编号BC005189
基因名称 MED4
Gene ID (NCBI) 29079
RRIDAB_2918601
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED4 is a component of the Meditor complex, which is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Meditor functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It also have an alternative name called "Activator-recruited cofactor 36 kDa component (ARC36) ".

实验方案

Product Specific Protocols
WB protocol for MED4 antibody 67839-1-IgDownload protocol
IHC protocol for MED4 antibody 67839-1-IgDownload protocol
IF protocol for MED4 antibody 67839-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols