• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67839-1-Ig (same clone as 67839-1-PBS)

    WB analysis using 67839-1-Ig (same clone as 67839-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67839-1-Ig (MED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67839-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67839-1-Ig (same clone as 67839-1-PBS)

    IHC staining of human breast cancer using 67839-1-Ig (same clone as 67839-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67839-1-Ig (MED4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67839-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67839-1-Ig (same clone as 67839-1-PBS)

    IF Staining of HepG2 using 67839-1-Ig (same clone as 67839-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED4 antibody (67839-1-Ig, Clone: 2E6F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue). This data was developed using the same antibody clone with 67839-1-PBS in a different storage buffer formulation.

MED4 Monoclonal antibody, PBS Only

MED4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67839-1-PBS

产品说明书

CloneNo. 2E6F4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ARC36, DRIP36, FLJ10956, HSPC126, MED4, mediator complex subunit 4, RP11 90M2.2, TRAP36, VDRIP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

67839-1-PBS targets MED4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, rat
免疫原 MED4 fusion protein Ag8562 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mediator complex subunit 4
别名 ARC36, DRIP36, FLJ10956, HSPC126, MED4, mediator complex subunit 4, RP11 90M2.2, TRAP36, VDRIP
计算分子量 270 aa, 30 kDa
观测分子量~36 kDa
GenBank蛋白编号BC005189
基因名称 MED4
Gene ID (NCBI) 29079
RRIDAB_2918601
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPJ6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MED4 is a component of the Meditor complex, which is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Meditor functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It also have an alternative name called "Activator-recruited cofactor 36 kDa component (ARC36) ".