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GSTM1 Monoclonal antibody

GSTM1 Monoclonal Antibody for WB, ELISA, FC (Inrta)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, chicken, mouse, rat, pig

Applications

WB, ELISA, FC (Inrta)

Conjugate

Unconjugated

CloneNo.

3C5C2

Cat No : 67862-1-Ig

Print datasheet

Synonyms

GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1, EC:2.5.1.18

验证数据展示

  • WB analysis of rat liver using 67862-1-Ig

    WB analysis of rat liver using 67862-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 67862-1-Ig

    FC experiment of HeLa using 67862-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GSTM1 (67862-1-Ig, Clone:3C5C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inrat liver tissue, HeLa cells, Jurkat cells, pig liver tissue, mouse liver tissue, chicken liver tissue
Positive FC (Inrta) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INRTA)FC (INRTA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67862-1-Ig targets GSTM1 in WB, ELISA, FC (Inrta) applications and shows reactivity with Human, chicken, mouse, rat, pig samples.

Tested Applications WB, ELISA, FC (Inrta) Application Description
Tested Reactivity Human, chicken, mouse, rat, pig
Immunogen GSTM1 fusion protein Ag3113 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutathione S-transferase mu 1
Synonyms GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1, EC:2.5.1.18
Calculated Molecular Weight 181 aa, 21 kDa
Observed Molecular Weight26-28 kDa
GenBank Accession NumberBC024005
Gene Symbol GSTM1
Gene ID (NCBI) 2944
RRIDAB_2918620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09488
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSTM1 antibody 67862-1-IgDownload protocol
FC protocol for GSTM1 antibody 67862-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols