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验证数据展示

  • IF Staining of HeLa using CL488-67862

    IF Staining of HeLa using CL488-67862

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 GSTM1 antibody (CL488-67862, Clone: 3C5C2 ) at dilution of 1:600, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-67862

    FC experiment of HeLa using CL488-67862

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human GSTM1 (CL488-67862, Clone:3C5C2) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated GSTM1 Monoclonal antibody

GSTM1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67862

Print datasheet

CloneNo. 3C5C2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, chicken

Applications

IF/ICC, FC (Intra)

Synonyms

GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1, EC:2.5.1.18

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67862-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67862 targets GSTM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig, chicken
Immunogen GSTM1 fusion protein Ag3113 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutathione S-transferase mu 1
Synonyms GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1, EC:2.5.1.18
Calculated Molecular Weight 181 aa, 21 kDa
Observed Molecular Weight26-28 kDa
GenBank Accession NumberBC024005
Gene Symbol GSTM1
Gene ID (NCBI) 2944
RRIDAB_2934578
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09488
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GSTM1 antibody CL488-67862Download protocol
Standard Protocols
Click here to view our Standard Protocols