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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 67886-1-Ig

    WB analysis using 67886-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of SW 1990 using 67886-1-Ig

    FC experiment of SW 1990 using 67886-1-Ig

    1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug Anti-HUMAN KDM1B (67886-1-Ig, Clone:1E1A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

KDM1B Monoclonal antibody

KDM1B Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67886-1-Ig
CloneNo. 1E1A3

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

AOF1, LSD2, C6orf193, 1E1A3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, K-562 cells, C6 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells
Positive FC (Intra) detected inSW 1990 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67886-1-Ig targets KDM1B in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KDM1B fusion protein Ag30672 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name amine oxidase (flavin containing) domain 1
Synonyms AOF1, LSD2, C6orf193, 1E1A3
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberNM_001364614
Gene Symbol KDM1B
Gene ID (NCBI) 221656
RRIDAB_2918643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NB78
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KDM1B antibody 67886-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols