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验证数据展示

  • FC experiment of SW 1990 using CL488-67886

    FC experiment of SW 1990 using CL488-67886

    1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-HUMAN KDM1B (CL488-67886, Clone:1E1A3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated KDM1B Monoclonal antibody

KDM1B Monoclonal Antibody for FC (Intra)
Cat No. CL488-67886
CloneNo. 1E1A3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

FC (Intra)

Synonyms

AOF1, LSD2, C6orf193, 1E1A3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inSW 1990 cells
For other applications, we recommend the unconjugated version of this antibody, 67886-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67886 targets KDM1B in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, rat, mouse
Immunogen KDM1B fusion protein Ag30672 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name amine oxidase (flavin containing) domain 1
Synonyms AOF1, LSD2, C6orf193, 1E1A3
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberNM_001364614
Gene Symbol KDM1B
Gene ID (NCBI) 221656
RRIDAB_2923817
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NB78
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 KDM1B antibody CL488-67886Download protocol
Standard Protocols
Click here to view our Standard Protocols