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验证数据展示

  • WB analysis using 67924-1-Ig

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HepG2 using 67924-1-Ig

    WB analysis of HepG2 using 67924-1-Ig

    WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HepG2 cells.

  • IHC staining of mouse cerebellum using 67924-1-Ig

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FARSB antibody included
  • IHC staining of mouse cerebellum using 67924-1-Ig

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FARSB antibody included
  • IF Staining of HeLa using 67924-1-Ig

    IF Staining of HeLa using 67924-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67924-1-Ig

    WB analysis of HeLa using 67924-1-Ig

    WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HeLa cells.

  • WB analysis using 67924-1-Ig

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Featured Product
  • KD/KO验证

FARSB Monoclonal antibody

FARSB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67924-1-Ig

产品说明书

CloneNo. 1H10C2
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat and More (2)

应用

WB, IHC, IF/ICC, ELISA

别名

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U-251 cells
Planning an IHC experiment? We recommend our IHCeasy FARSB Ready-To-Use IHC Kit. FARSB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

67924-1-Ig targets FARSB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 Human, Mouse, Rat
文献引用反应性human, mouse
免疫原 FARSB fusion protein Ag9695 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 phenylalanyl-tRNA synthetase, beta subunit
别名 FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS
计算分子量 589 aa, 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC017783
基因名称 FARSB
Gene ID (NCBI) 10056
RRIDAB_2918676
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NSD9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FARSB antibody 67924-1-IgDownload protocol
IHC protocol for FARSB antibody 67924-1-IgDownload protocol
IF protocol for FARSB antibody 67924-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC

Nan Fang Yi Ke Da Xue Xue Bao

[FARSB stratifies prognosis and cold tumor microenvironment across different cancer types: an integrated single cell and bulk RNA sequencing analysis]

Authors - Ziran Zhang
View Article