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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FARSB Monoclonal antibody

FARSB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H10C2

Cat no : CL488-67924

Print datasheet

Synonyms

Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB

验证数据展示

  • IF Staining of U-251 using CL488-67924

    IF Staining of U-251 using CL488-67924

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using CoraLite® Plus 488 FARSB antibody (CL488-67924, Clone: 1H10C2 ) at dilution of 1:200.

  • FC experiment of U-251 using CL488-67924

    FC experiment of U-251 using CL488-67924

    1x10^6 U-251 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated FARSB Monoclonal antibody (CL488-67924, Clone:1H10C2)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of U-251 using CL488-67924

    IF Staining of U-251 using CL488-67924

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using CoraLite® Plus 488 FARSB antibody (CL488-67924, Clone: 1H10C2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy FARSB Ready-To-Use IHC Kit. FARSB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67924-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67924 targets FARSB in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen FARSB fusion protein Ag9695 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phenylalanyl-tRNA synthetase, beta subunit
Synonyms Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB
Calculated Molecular Weight 589 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC017783
Gene Symbol FARSB
Gene ID (NCBI) 10056
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NSD9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FARSB antibody CL488-67924Download protocol
FC protocol for CL Plus 488 FARSB antibody CL488-67924Download protocol
Standard Protocols
Click here to view our Standard Protocols