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FARSB Monoclonal antibody

FARSB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H10C2

Cat no : 67924-1-Ig

Print datasheet

Synonyms

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS

验证数据展示

  • WB analysis using 67924-1-Ig

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HepG2 using 67924-1-Ig

    WB analysis of HepG2 using 67924-1-Ig

    WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HepG2 cells.

  • IHC staining of mouse cerebellum using 67924-1-Ig

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FARSB antibody included
  • IHC staining of mouse cerebellum using 67924-1-Ig

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FARSB antibody included
  • IF Staining of HeLa using 67924-1-Ig

    IF Staining of HeLa using 67924-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67924-1-Ig

    WB analysis of HeLa using 67924-1-Ig

    WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HeLa cells.

  • WB analysis using 67924-1-Ig

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U-251 cells
Planning an IHC experiment? We recommend our IHCeasy FARSB Ready-To-Use IHC Kit. FARSB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

67924-1-Ig targets FARSB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Immunogen FARSB fusion protein Ag9695 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phenylalanyl-tRNA synthetase, beta subunit
Synonyms FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS
Calculated Molecular Weight 589 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC017783
Gene Symbol FARSB
Gene ID (NCBI) 10056
RRIDAB_2918676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NSD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FARSB antibody 67924-1-IgDownload protocol
IHC protocol for FARSB antibody 67924-1-IgDownload protocol
IF protocol for FARSB antibody 67924-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC

Nan Fang Yi Ke Da Xue Xue Bao

[FARSB stratifies prognosis and cold tumor microenvironment across different cancer types: an integrated single cell and bulk RNA sequencing analysis]

Authors - Ziran Zhang
View Article