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验证数据展示

  • WB analysis using 67930-1-Ig

    WB analysis using 67930-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67930-1-Ig (DARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat cerebellum using 67930-1-Ig

    IHC staining of rat cerebellum using 67930-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67930-1-Ig (DARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DARS1 antibody included
  • IF Staining of SH-SY5Y using 67930-1-Ig

    IF Staining of SH-SY5Y using 67930-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DARS antibody (67930-1-Ig, Clone: 3C5D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DARS Monoclonal antibody

DARS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67930-1-Ig

产品说明书

CloneNo. 3C5D3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy DARS1 Ready-To-Use IHC Kit. DARS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67930-1-Ig targets DARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 DARS fusion protein Ag7059 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 aspartyl-tRNA synthetase
别名 Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202
计算分子量 57 kDa
观测分子量50-57 kDa
GenBank蛋白编号BC000629
基因名称 DARS
Gene ID (NCBI) 1615
RRIDAB_2918682
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14868
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DARS antibody 67930-1-IgDownload protocol
IHC protocol for DARS antibody 67930-1-IgDownload protocol
IF protocol for DARS antibody 67930-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols