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CoraLite® Plus 488-conjugated DARS Monoclonal antibody

DARS Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C5D3

Cat No : CL488-67930

Print datasheet

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202

验证数据展示

  • IF Staining of HeLa using CL488-67930

    IF Staining of HeLa using CL488-67930

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 DARS antibody (CL488-67930, Clone: 3C5D3 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DARS1 Ready-To-Use IHC Kit. DARS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67930-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67930 targets DARS in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen DARS fusion protein Ag7059 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name aspartyl-tRNA synthetase
Synonyms Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202
Calculated Molecular Weight 57 kDa
Observed Molecular Weight50-57 kDa
GenBank Accession NumberBC000629
Gene Symbol DARS
Gene ID (NCBI) 1615
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14868
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DARS antibody CL488-67930Download protocol
FC protocol for CL Plus 488 DARS antibody CL488-67930Download protocol
Standard Protocols
Click here to view our Standard Protocols