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验证数据展示

  • WB analysis using 67976-1-Ig

    WB analysis using 67976-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67976-1-Ig (RNASEH2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 67976-1-Ig

    IHC staining of human skin cancer using 67976-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67976-1-Ig (RNASEH2A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RNASEH2A Monoclonal antibody

RNASEH2A Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67976-1-Ig

产品说明书

CloneNo. 1A2E8

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, ELISA

别名

ribonuclease H2, subunit A, Ribonuclease H2 subunit A, JUNB, EC:3.1.26.4, Aicardi-Goutieres syndrome 4 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Hela cells, MCF-7 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67976-1-Ig targets RNASEH2A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 RNASEH2A fusion protein Ag9256 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ribonuclease H2, subunit A
别名 ribonuclease H2, subunit A, Ribonuclease H2 subunit A, JUNB, EC:3.1.26.4, Aicardi-Goutieres syndrome 4 protein
计算分子量 299 aa, 33 kDa
GenBank蛋白编号BC011748
基因名称 RNASEH2A
Gene ID (NCBI) 10535
RRIDAB_2918725
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75792
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).

实验方案

Product Specific Protocols
WB protocol for RNASEH2A antibody 67976-1-IgDownload protocol
IHC protocol for RNASEH2A antibody 67976-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols