GPHN Monoclonal antibody
GPHN Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1D7C4
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67995-1-Ig targets GPHN in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human |
| Immunogen | GPHN fusion protein Ag23708 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | gephyrin |
| Synonyms | Domain E, GEPH, gephyrin, GPH, GPHN, GPHRYN, KIAA1385, MPT adenylyltransferase, MPT Mo transferase |
| Calculated Molecular Weight | 769 aa, 83 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | BC030016 |
| Gene Symbol | GPHN |
| Gene ID (NCBI) | 10243 |
| RRID | AB_2918744 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NQX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPHN antibody 67995-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |